Invertebrate reference gene standards
£80 per single assay or select any three assays for £210
To order please send a Purchase Order to info@qstandard.co.uk
Drosophila melanogaster
Gene symbol |
Accession number(s) |
Product code |
Amplicon length (bp) |
Intron: span/flank |
Intron length (bp) |
Amplicon %GC |
| Act5c | NM_167053, NM_078497, NM_001014726, NM_001014725 | dme_Act5c_001 | 111 |
no |
n/a |
64.0 |
| eIF-4a | NM_164668, NM_164669, NM_057247, NM_164670 | dme_eIF-4a_001 | 165 |
span |
n/a |
55.6 |
| Gapdh2 | NM_080714, NM_080352 | dme_Gapdh2_001 | 70 |
no |
n/a |
48.6 |
Caenorhabditis elegans
Gene symbol |
Wormbase sequence name |
Product code |
Amplicon length (bp) |
Intron: span/flank |
Intron length (bp) |
Amplicon %GC |
| cdc-42 | R07G3.1 | cel_cdc-42_001 | 129 |
flank |
53 |
48.1 |
| pmp-3 | C54G10.3 | cel-pmp-3_001 | 73 |
flank |
51 |
49.6 |
| Y45F10D.4 | Y45F10D.4 | cel_Y45F10D.4_001 | 83 |
span |
n/a |
50.0 |
- Primers have been designed to avoid 3' SNPs and secondary structure (self-annealing)
- No secondary structure in the amplicon for any assay (positive delta G)
- Specificity has been checked by melt peak analysis and the presence of only a single band on a gel
- Standard curves down to 10 copies per reaction have been run for each assay and all show excellent linearity (R2 >0.99) Efficiency (>95%) and sensitivity (repeat of 10 copies consistently detected)
- Primers have been tested at an annealing temperature of 57oC. Higher annealing temperatures (up to 60oC) are possible, but require individual validation.
