Human reference gene standards

£80 per single assay or select any five assays for £350

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Gene symbol	Accession number(s)	Product code	Amplicon length (bp)	Intron: span/flank	Intron length (bp)	Amplicon %GC
ACTB	NM_001101	hsa_ACTB_002	117	flank	110	55.6
ACTB	NM_001101	hsa_ACTB_003	212	flank & span	n/a	68.6
B2M	NM_004048	hsa_B2M_002	112	flank	3807	46.4
G6PD	NM_021130 & NM_001042351	hsa_G6PD_002	80	flank	157	55.0
GAPDH	NM_002046	hsa_GAPDH_001	87	span	n/a	54.0
GNB2L1	NM_006098	hsa_GNB2L1_001	224	flank	2218	52.7
HMBS	NM_000190 & NM_001024382	hsa_HMBS_002	90	flank	1147	57.8
HPRT1	NM_000194	hsa_HPRT1_002	127	span	n/a	43.3
PLA2G6	NM_003560	hsa_PLA2G6_003	181	span	n/a	59.6
PPIA	NM_021130	hsa_PPIA_002	218	flank & span	n/a	50.5
RPL13	NM_000994	hsa_RPL13_001	81	span	n/a	48.2
RPL32	NM_000994.3 & NM_033251	hsa_RPL32_001	152	span	n/a	51.3
RPLP0	NM_001002 & NM_053275	hsa_RPL32_001	88	flank	1088	51.1
SDHA	NM_004168	hsa_SDHA_002	93	flank	1890	54.8
TBP	NM_003194	hsa_TBP_002	87	flank	4707	57.5
TUBB	NM_178014	hsa_TUBB_001	115	flank	1654	47.0
YWHAZ	NM_003406, NM_145690, NM_001135699, NM_001135700, NM_001135701, & NM_001135702	hsa_YWHAZ_001	94	no	n/a	45.1

Primers have been designed to avoid 3' SNPs and secondary structure (self-annealing)
No secondary structure in the amplicon for any assay (positive delta G)
Specificity has been checked by melt peak analysis and the presence of only a single band on a gel
Standard curves down to 10 copies per reaction have been run for each assay and all show excellent linearity (R² >0.99) Efficiency (>95%) and sensitivity (repeat of 10 copies consistently detected)
Primers have been tested at an annealing temperature of 57^oC. Higher annealing temperatures (up to 60^oC) are possible, but require individual validation.