Rodent reference gene standards
£80 per single assay or select any five assays for £350
To order please send a Purchase Order to info@qstandard.co.uk
Mouse
Gene symbol |
Accession number(s) |
Product code |
Amplicon length (bp) |
Intron: span/flank |
Intron length (bp) |
Amplicon %GC |
| Actb | NM_007393 | mmu_Actb_001 | 186 |
span |
n/a |
55.7 |
| B2m | NM_009735 | mmu_B2m_001 | 71 |
span |
n/a |
53.5 |
| Gapdh | NM_08084 | mmu_Gapdh_001 | 133 |
span |
n/a |
53.4 |
| Hmbs | NM_013551 & NM_001110251 | mmu_Hmbs_001 | 73 |
span |
n/a |
49.3 |
| Hprt1 | NM_013556 | mmu_Hprt_001 | 91 |
span |
n/a |
62.6 |
| Pla2g12a | NM_023196 | mmu_Pla2g12a_001 | 187 |
flank |
11440 |
56.7 |
| Rpl13 | NM_023281 | mmu_Rpl13_002 | 133 |
span |
340 |
60.9 |
| Sdha | NM_016738 | mmu_Sdha_001 | 85 |
no |
n/a |
51.2 |
| Tbp | NM_013684 | mmu_Tbp_001 | 232 |
span & flank |
n/a |
47.4 |
Rat
Gene symbol |
Accession number(s) |
Product code |
Amplicon length (bp) |
Intron: span/flank |
Intron length (bp) |
Amplicon %GC |
| Actb | NM_031144 | rno_Actb_001 | 300 |
flank |
214 |
68.6 |
| Gapdh | NM_017008 | rno_Gapdh_001 | 310 |
span & flank |
n/a |
52.6 |
| Hprt1 | NM_012583 | rno_Hprt1_001 | 94 |
span |
n/a |
45.8 |
| Rpl13 | NM_031101 | rno_Rpl13_001 | 76 |
span |
n/a |
61.8 |
| Sdha | NM_130482 | rno_Sdha_001 | 88 |
flank |
2846 |
52.3 |
| Tbp | NM_001004198 | rno_Tbp_001 | 75 |
span |
n/a |
41.3 |
- Primers have been designed to avoid 3' SNPs and secondary structure (self-annealing)
- No secondary structure in the amplicon for any assay (positive delta G)
- Specificity has been checked by melt peak analysis and the presence of only a single band on a gel
- Standard curves down to 10 copies per reaction have been run for each assay and all show excellent linearity (R2 >0.99) Efficiency (>95%) and sensitivity (repeat of 10 copies consistently detected)
- Primers have been tested at an annealing temperature of 57oC. Higher annealing temperatures (up to 60oC) are possible, but require individual validation.
