S E R V I C E S
Services and Products
Email: info@qstandard.co.uk
Reference gene standards for RT-qPCR
We have a number of validated, pre-designed assays for commonly-used reference genes. All our assays have been thoroughly tested on complex biological samples (i.e cell or tissue cDNA samples) in real qPCR reactions to ensure:
● specificity - a single band of the correct size is generated, a single melt peak is observed
● sensitivity – high efficiency ensures that as few as 10 copies can be measured in complex biological samples
● linearity - standard curves from 107-101 copies per reaction are linear, r2>0.99
● good efficiency - ≥95% calculated from the slope of the standard curve
You will receive:
● 1x109 copies of purified standard (sufficient for up to 100 standard curves) and a complimentary aliquot of each primer (while stocks last). ● full disclosure of all primer and amplicon sequences, positions, and full quality assurance documentation (standard curve for 107-101 copies copies/reaction, product melt curve and agarose gel image).
To buy, send a purchase order to info@qstandard.co.uk
Click on the links below to view assay details and product codes (links open in a new window):
human, mouse & rat, D. melanogaster, C. elegans, Xenopus
Price: £80 per standard or £350 for a set of five standards of your choice - free delivery within the UK
How many reference genes should I run to normalise my expression data? MIQE guidelines suggest more than one reference gene should be evaluated. We recommend running a panel of genes and using software such as geNorm, Normfinder or BestKeeper to identify the most stable which may then be used to normalise data. Once the most stable reference genes are established for a given set of experimental conditions, only those reference genes need be run in subsequent experiments.
Custom gene standards for RT-qPCR
Want to do absolute quantification but can't face making standards? We can provide custom-made known copy number standards for RT-qPCR. Our comprehensive service includes assay design, synthesis and full experimental validation, provision of quality assurance data and disclosure of all sequences.
Provide us with an accession number for your gene of interest (we may require a small amount of cDNA) and we will design an assay, make the standards and provide you with 1x109 copies of purified standard (sufficient for up to 100 standard curves) plus the primer sequences. Prior to despatch we will check the assay specifity by melt curve and agarose gel analysis, the linearity from 107-101 copies (r2>0.99), and assay efficiency (≥95%). These quality control data will be included with your standards.
Price: £90 per standard - free delivery within the UK
To order please email us with your requirements at info@qstandard.co.uk
qPCR services
We are also able to provide qPCR services including:
- RNA quality controls
- Reverse transcription
- PCR inhibition check
- gDNA contamination check
- qPCR (cDNA or DNA)
- Normalisation against a panel of reference genes
Costs are calculated according to the number of assays required and the number of samples to be run. A standard set-up fee of £100 applies irrespective of assay or sample number. Contact us for a quotation.